Research Journal of Pharmacognosy and Phytochemistry

V. Sreenivasulu ¹, K. N. Jayaveera ², P. Mallikarjuna Rao ³

Affiliations
1 Dept. of Biotechnology, KLR Pharmacy College, Paloncha-507115, Khammam- District, A.P, IN
2 Oil Technological Research Institute, JNT University, Anantapur–15001, A.P, IN
3 International Medical University, Kualalumpur, -57000, MY

Abstract

Production of L-asparaginase employing Aspergillus sp. VEM-9 under solid-state fermentation was optimized. Different substrates like rice bran, green gram bran, wheat rawa, wheat bran, Bombay rawa, black gram bran, barley, saw dust, jowar flour, rice flour, castor oil cake, ground nut oil cake, coconut oil cake, sesame oil cake were studied to optimize the best substrate. Groundnut oil cake showed the highest enzyme yield. Different physical fermentation factors were optimized. The maximum productivity of L-asparaginase (60 U/gds) was achieved by employing groundnut oil cake and optimized process parameters including incubation period of 5 days, initial moisture content of solid substrate 90%, 1: 10 (v/w) ratio of salt solution to weight of groundnut oil cake, inoculum level 20%(v/w), incubation temperature at 30°C and initial pH 6.5.

Keywords

L-Asparaginase, Aspergillus sp. VEM-9, Groundnut Oil Cake, Optimization, Solid-State Fermentation.

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V. Sreenivasulu ¹, K. N. Jayaveera ², P. Mallikarjuna Rao ³

Affiliations
1 Dept. of Biotechnology, KLR Pharmacy College, Paloncha-507115,Khammam- District, A.P, IN
2 Oil Technological Research Institute, JNT University, Anantapur–15001, A.P, IN
3 International Medical University, Kualalumpur, 57000, MY

Abstract

The extracellular L-asparaginase production by fungi isolated from soil samples by using pH and dye based method. Various physical and chemical parameters were optimized under submerged fermentation for Lasparaginase production. Maximum productivity of L-asparaginase (19.5 U/ml) was achieved by employing medium containing 2% (w/v) Lasparagine as substrate concentration, 1%(w/v) glucose as carbon source, 1% (w/v) ammonium sulphate as an additional nitrogen source with the incubation period of 96 h and incubation temperature at 30°C, initial pH 6.5 at an inoculum level 20% (v/v) with 48 h old inoculum was found to be optimum for maximum yield.

Keywords

L-Asparaginase, Optimization and Submerged Fermentation.

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